Peptide Occurring in Enterobacteriaceae Triggers Streptococcus pneumoniae Cell Death

Peptide Occurring in Enterobacteriaceae Triggers Streptococcus pneumoniae Cell Death

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Non-encapsulated Streptococcus pneumoniae often possess two genes, aliB-like ORF 1 and aliB-like ORF 2, in place of capsule genes.AliB-like ORF 1 is thought to encode a substrate binding protein of an ABC transporter which binds peptide SETTFGRDFN, found in 50S ribosomal subunit protein L4 of Enterobacteriaceae.Here, we investigated the effect of binding of AliB-like ORF 1 peptide on the transcriptome and proteome of non-encapsulated pneumococci.We found upregulation of gene expression of a metacaspase and a gene encoding us polo assn mens sweaters N-acetylmuramoyl-L-alanine amidase, both of which are proposed to be involved in programmed cell death in prokaryotic cells.Proteome profiling indicated upregulation of transcriptional regulators and downregulation of metabolism-associated genes.

Exposure to the peptide specifically triggered death in pneumococci which express AliB-like ORF 1, with the bacteria having an apoptotic appearance click here by electron microscopy.We propose that binding of the AliB-like ORF 1 peptide ligand by the pneumococcus signals a challenging environment with hostile bacterial species leading to death of a proportion of the pneumococcal population.